Hyaluronectin blocks the stimulatory effect of hyaluronan‐derived fragments on endothelial cells during angiogenesis in vitro

FEBS Letters

Delpech, Bertrand²  Mabilat-Pragnon, Christelle³  Trochon, Véronique³  Soria, Claudine³  Legrand, Yves³  Bertrand, Philippe²  Soria, Jeannette¹  Lu, He³ 

[1] Laboratoire Sainte Marie, Hôtel Dieu, Paris, France;Laboratoire d'Oncologie Moléculaire, Centre Henri Becquerel, Rouen, France;INSERM U353, Bat. INSERM, Institut d'Hématologie, Hôpital Saint Louis, 1 Ave Claude Vellefaux, F-75475 Paris Cedex 10, France

关键词: Angiogenesis;    Endothelial cell;    Hyaluronectin;    Hyaluronan;    Hyaluronidase;   

DOI  :  10.1016/S0014-5793(97)01337-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hyaluronic acid (HA) is a glycosaminoglycan of the extracellular matrix. Its fragmentation by the hyaluronidase, secreted by tumor cells, facilitates tumor invasion and the HA degradation products generated stimulate angiogenesis. We report here that the HA-binding protein hyaluronectin (HN) inhibits the stimulatory effect of HA-derived fragments on the proliferation and migration of endothelial cells in vitro, and hampers the organization of endothelial cells into capillary-like structures. Since HN strongly inhibits endothelial cell adhesion to immobilized HA, it is postulated that HN acts by impairing the binding to endothelial cells of HA fragments generated by hyaluronidase, thereby neutralizing the effect of HA degradation products on angiogenesis. Our results reveal a new mechanism by which the angiogenesis induced by HA fragments is modulated by HN.

【 授权许可】

Unknown