Changes in anion permeability following hypotonic challenge in rat brain endothelial cells: different responses in primary cultures and in immortalised RBE4 cells

FEBS Letters

von Weikersthal, S.F¹  Barrand, M.A¹  Wright, K.A¹  Roux, F.A²  Seetharaman, S¹ 

[1] Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QJ, UK;INSERM U26, Hôpital F. Widal, 75010 Paris, France

关键词: Iodide efflux;    Anion permeability;    Primary culture;    Immortalised rat brain endothelial cells;   

DOI  :  10.1016/S0014-5793(97)01320-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hypotonicity-induced anion permeability changes were investigated but not detected in immortalised (RBE4) rat brain endothelial cells using iodide efflux measurements. Large, rapid increases were however observed in primary cultured cells. Both cell types were reinvestigated following culture in a common growth factor-depleted medium. Responses were still undetectable in the immortalised RBE4 cells. Reduced responses were observed in the primary cultured cells that also showed altered morphology and decreased activity of another transporter, P-glycoprotein. Thus both immortalisation and different culture conditions may alter functional expression in these cells of transporters involved in hypotonicity-induced anion permeability changes.

【 授权许可】

Unknown