Inhibition of electrogenic anion entry into rat liver mitochondria by N,N'‐dicyclohexylcarbodiimide

FEBS Letters

Warhurst, Ian W.¹  Selwyn, Michael J.¹  Dawson, Alan P.¹ 

[1] School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, England

关键词: Anion permeability;    Mitochondria;    Uncoupling protein;    Dicyclohexylcarbodiimide;    Membrane potential;    pH gradient;    DCCD;    N;    N'-dicyclohexylcarbodiimide;    CCCP;    carbonyl cyanide-m-chlorophenyl hydrazone;    FCCP;    carbonylcyanide-p-trifluoromethoxy phenylhydrazone;    EGTA;    ethyleneglycol-bis-(β-aminoethylether)-N;    N'-tetraacetic acid;    HEDTA;    N-(2-hydroxyethyl)ethylenediamine-N;    N';    N'-triacetic acid;    HEPES;    4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid;   

DOI  :  10.1016/0014-5793(82)81110-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The carboxyl group reagent dicyclohexylcarbodiimide inhibits the electrogenic entry of Cl⁻ and NO₃ ⁻ into rat liver mitochondria at alkaline pH. The inhibition is time dependent and 50% inhibition is obtained by the addition of 3–4 nmol DCCD/mg protein. The blockage of the pH-dependent anion-conducting pore appears to be unrelated to the other known actions of DCCD on rat liver mitochondria but seems similar to its effect on the uncoupling protein of brown adipose tissue.

【 授权许可】

Unknown