Localization of metallothionein in nuclei of growing primary cultured adult rat hepatocytes

FEBS Letters

Shimizu, Nobuyoshi³  Fukuyama, Ryuichi³  Tsujikawa, Kazutake¹  Kimura, Masami²  Mimura, Tsutomu¹  Imai, Takumi¹  Kayamori, Yuzo¹  Otaki, Noriko²  Kakutani, Makoto¹ 

[1] Faculty of Pharmaceutical Sciences, Osaka University, I-6 Yamadaoka, Suita, Osaka 565, Japan;National Institute of Industrial Health, 6-21-1 Nagao, Tama, Kawasaki, Kanagawa 211, Japan;Department of Molecular Biology, Kelb University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160, Japan

关键词: Metallothionein;    Nucleus;    Hepatocyte;    Primary culture;    EGF;    epidermal growth factor;    MT;    metallothionein;   

DOI  :  10.1016/0014-5793(91)80597-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In primary cultured adult rat hepatocytes stimulated by epidermal growth factor and insulin, dramatic changes in the subcellular distribution of metallothionein were clarified by indirect immunofluorescence using antisera specific for this protein. Metallothionein was detected only in the cytoplasm of cultured hepatocytes in the G₀ and G₁ phases, but was concentrated in the cell nuclei in the early S phase. The strongest staining pattern in the nuclei was observed 12 h after stimulation. Subsequently, the intensity of metallothionein staining in the nuclei decreased. These results suggests that primary cultured hepatocytes are suitable for examining the relation between subcellular localization of metallothionein and cell growth.

【 授权许可】

Unknown