Glycogenin, the primer of glycogen synthesis, binds to actin

FEBS Letters

Ferrer, Juan C¹  Guinovart, Joan J¹  Baqué, Susanna¹ 

[1] Departament de Bioquı́mica i Biologia Molecular, Universitat de Barcelona, Martı́ i Franquès 1, E-08028 Barcelona, Spain

关键词: Glycogenin;    Glycogen metabolism;    Actin cytoskeleton;    Green fluorescent protein;    GFP;    green fluorescent protein;   

DOI  :  10.1016/S0014-5793(97)01299-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have studied the intracellular localization of glycogenin by fusing green fluorescent protein (GFP) to the N-terminus of rabbit muscle glycogenin and expressing the chimeric protein in C₂C₁₂, COS-1 and rat hepatic cells. The fusion protein showed a nuclear and cytosolic distribution and partially co-localized with actin in the cytosol. Disruption of the actin cytoskeleton with cytochalasin D led to a change in the pattern of green fluorescence, which coincided with that observed for the remaining non-depolymerized actin. The distribution of the single point mutant K324A was completely uniform and was not affected by this drug. These findings indicate that rabbit muscle glycogenin binds to actin through the heptapeptide ³²¹DNIKKKL³²⁷, a common motif found in other actin-binding proteins, which is located at the C-terminal end of this protein, and suggest that the actin cytoskeleton plays an important role in glycogen metabolism.

【 授权许可】

Unknown