Inhibitory effect of curcumin on mammalian phospholipase D activity

FEBS Letters

Nishijima, Masahiro¹  Yamamoto, Hisanori¹  Kawasaki, Kiyoshi¹  Hanada, Kentaro¹ 

[1] Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162, Japan

关键词: Curcumin;    Phospholipase D;    TPA;    12-O-tetradecanoylphorbol-13-acetate;    TNF;    tumor necrosis factor;    FCS;    fetal calf serum (heat-inactivated);    PC;    phosphatidylcholine;    PA;    phosphatidic acid;    PBut;    phosphatidyl butanol;    PC-PLC;    PC-specific phospholipase C;    PLD;    phospholipase D;    G proteins;    guanine-nucleotide binding proteins;    ARF;    ADP-ribosylation factor;    GTPγS;    guanosine 5′-O-[γ-thio]triphosphate;    DMSO;    dimethylsulfoxide;    PDTC;    pyrrolidine dithiocarbamate;   

DOI  :  10.1016/S0014-5793(97)01280-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Curcumin, the major yellow pigment of turmeric (Curcuma longa), has strong anti-carcinogenic and anti-inflammatory activities. We examined the effects of curcumin on enzyme activities of the following phospholipases in a cell-free system: G protein-mediated phospholipase D (PLD), phosphatidylinositol-specific phospholipase C, and phospholipase A₂ from mouse macrophage-like cell line J774.1 cells, sphingomyelinase from bovine brain, and phosphatidylcholine-phospholipase C from Bacillus cereus. Curcumin inhibited several types of phospholipases, most effectively PLD among those tested. It also inhibited 12-O-tetradecanoylphorbol-13-acetate-induced PLD activation in intact J774.1 cells in a dose-dependent manner. These results suggest that the anti-inflammatory and anti-carcinogenic action of curcumin is partly due to the inhibition of PLD.

【 授权许可】

Unknown