Cloning of a functional vesicular GABA and glycine transporter by screening of genome databases

FEBS Letters

Giros, Bruno²  Hamon, Michel²  Isambert, Marie-Françoise¹  El Mestikawy, Salah²  Gasnier, Bruno¹  Sagné, Corinne¹  Henry, Jean-Pierre¹ 

[1] CNRS ERS 575, Institut de Biologie Physico-Chimique, 13 rue P. et M. Curie, 75005 Paris, France;INSERM U 288, Faculté de Médecine Pitié-Salpêtrière, 91 bd de l'Hôpital, 75634 Paris Cedex 13, France

关键词: Neurotransmission;    Synaptic vesicle;    Neurotransmitter transporter;    GABA;    Glycine;    Caenorhabditis elegans;    EST;    expressed sequence tag;    GABA;    γ-aminobutyric acid;    GAD;    glutamic acid decarboxylase;    GAT;    plasma membrane GABA transporter;    GLYT;    plasma membrane glycine transporter;    RACE;    rapid amplification of cDNA ends;    TM;    transmembrane domain;    VIAAT;    vesicular inhibitory amino acid transporter;    VMAT;    vesicular monoamine transporter;   

DOI  :  10.1016/S0014-5793(97)01279-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The unc-47 locus of Caenorhabditis elegans has been suggested to encode a synaptic vesicle GABA transporter. Here we used hydropathy plot analysis to identify a candidate vesicular GABA transporter in genomic sequences derived from a region of the physical map comprising unc-47. A mouse homologue was identified and cloned from EST database information. In situ hybridization in rat brain revealed codistribution with both GABAergic and glycinergic neuronal markers. Moreover, expression in COS-7 and PC12 cells induced an intracellular, glycine-sensitive GABA uptake activity. These observations, consistent with previous data on GABA and glycine uptake by synaptic vesicles, demonstrate that the mouse clone encodes a vesicular inhibitory amino acid transporter.

【 授权许可】

Unknown