FEBS Letters | |
The recombinant GABA transporter GAT1 is downregulated upon activation of protein kinase C | |
Sato, Kohji1  Betz, Heinrich1  Schloss, Patrick1  | |
[1] Abteilung Neurochemie, Max-Planck-Institut für Hirnforschung, Deutschordenstrasse 46, D-60528 Frankfurt, Germany | |
关键词: GABA uptake; Neurotransmitter transporter; Protein kinase C; Rat; GABA; γ-aminobutyric acid; GAT; GABA transporter; H7; 1-(5-isoquinolinylsulphonyl)-2-methylpiperazine; HEK cell; human embryonic kidney cell; PMA; phorbol 12-myristate 13-acetate; PKC; protein kinase C; SERT; serotonin transporter; TPA; 12-0-tetradecanoylphorbol-13-acetate; PDD; 4α-phorbol-12; 13-dide-canoate; | |
DOI : 10.1016/0014-5793(95)01191-G | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Treatment of human embryonic kidney 293 cells expressing the rat γ-aminobutyric acid (GABA) transporter 1 (GAT1) with the protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) was found to decrease the velocity of specific [3H]GABA uptake. This downregulation varied with extracellular GABA concentration and was blocked by the PKC inhibitors 1-(5-isoquinolinylsulphonyl)-2-methylpiperazine (H7) and staurosporine. An about 50% reduction of uptake velocity by PMA treatment was observed at GABA concentrations > 1 μM, whereas only a minor effect was seen at low substrate concentrations. These data indicate that GAT1 activity is downregulated by PKC activation.
【 授权许可】
Unknown
【 预 览 】
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