Protein kinases and protein synthesis are involved in desensitization of the plasminogen activator response of rat Sertoli cells by follicle‐stimulating hormone

FEBS Letters

Guillou, F.¹  Combarnous, Y.¹  Laurent-Cadoret, V.¹ 

[1] Station de Physiologie de la Reproduction des Mammifères Domestiques PRMD, INRA/CNRS, URA 1291, 37380 Nouzilly, France

关键词: Desensitization;    Protein kinase cAMP dependent;    Protein kinase C;    Tissue-type plasminogen activator;    Follicle-stimulating hormone;    Sertoli cell;    Rat;   

DOI  :  10.1016/0014-5793(94)00906-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tissue-type plasminogen activator (tPA) secretion is a specific response of Sertoli cells to follicle-stimulating hormone (FSH), which is lower after preincubation of the cells with low FSH concentrations because of FSH receptor/G_s protein uncoupling. In this report, we present evidence that this desensitization induced by the lowest FSH concentrations is suppressed by specific peptidic inhibitors of endogenous PKA and PKC in permeabilized Sertoli cells. In contrast, desensitization promoted by slightly higher FSH concentrations is not mediated through PKA or PKC activation but is dependent on protein neosynthesis.

【 授权许可】

Unknown