Role of protein kinase C and carboxyl‐terminal region in acute desensitization of vasopressin V1a receptor

FEBS Letters

Preisser, Laurence¹  Corman, Bruno¹  Ancellin, Nicolas¹  Morel, Alain¹ 

[1] Service de Biologie Cellulaire, CEA, Centre d'Etudes de Saclay, 91191 Gif-sur-Yvette Cedex, France

关键词: Vasopressin V1a receptor;    Protein kinase C;    Desensitization;    Intracellular calcium;    Xenopus oocyte;   

DOI  :  10.1016/S0014-5793(97)00914-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The role of protein kinase C activation and carboxyl-terminal region in rapid desensitization of the vasopressin V1a receptor was investigated in Xenopus oocytes. Preincubation of the oocytes with vasopressin or with the diacylglycerol analog 1-oleoyl-2-acetyl-sn-glycerol (OAG), or direct injection of active protein kinase C, all blunted the calcium response of the V1a receptor. Truncation of the 51 terminal amino acids (S374STOP) modified neither the intracellular calcium response to vasopressin nor its desensitization by vasopressin or OAG. These data suggest that desensitization of the V1a receptor is mediated by PKC activation and that its carboxyl-terminal domain is not required for signal transduction and rapid desensitization.

【 授权许可】

Unknown