Expression and regulation of the bovine vesicular monoamine transporter gene

FEBS Letters

Gagnon, Jean¹  Isambert, Marie-Françoise²  Krejci, Eric³  Massoulié, Jean³  Gasnier, Bruno²  Sagné, Corinne²  Henry, Jean-Pierre²  Botton, Damien² 

[1] Institut de Biologie Structurale, 41 avenue des Martyrs, 38027 Grenoble Cedex 1, France;Service de Neurobiologie Physico-Chimique, CNRS Unité Associée 1112, Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France;Laboratoire de Neurobiologie, CNRS Unité Associée 295, Ecole Normale Supérieure, 46 rue d'Ulm, 75005 Paris, France

关键词: Chromaffin granule;    Neurotransmitter transporter;    Stimulation-secretion coupling;    Synaptic vesicle;   

DOI  :  10.1016/0014-5793(93)80432-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In monoaminergic cells, the neurotransmitter is accumulated into secretory or synaptic vesicles by a tetrabenazine- and reserpine-sensitive transporter, catalyzing an H⁺/monoamine antiport. The major vesicular monoamine transporter from bovine chromaffin cells was cloned, using sequences common to adrenal medulla and brain rat vesicular monoamine transporters. Its identity was confirmed by peptide sequences, determined from the purified protein. Surprisingly, the bovine adrenal medulla sequence, bVMAT₂, is more related to the transporter from human and rat brain than to that from rat adrenal medulla. PCR amplification showed that bVMAT₂, is expressed in both adrenal medulla and brain, in contrast with the situation reported in rats, where distinct genes appear to be expressed in brain (SVAT or MAT, now renamed rVMAT₂) and in the adrenal medulla (CGAT, now renamed rVMAT₁). In bovine chromaffin cells, long-term depolarization by KC1 resulted in an increase in the level of bVMAT₂ mRNA, in agreement with the previously observed increase in the transporter binding sites, suggesting that a coupling between stimulation, secretion and synthesis changes the composition of the secretory granule membrane.

【 授权许可】

Unknown