Identification of the yeast ACR1 gene product as a succinate‐fumarate transporter essential for growth on ethanol or acetate

FEBS Letters

Runswick, Michael J²  Walker, John E²  Palmieri, Luigi²  Lasorsa, Francesco M¹  De Palma, Annalisa¹  Palmieri, Ferdinando¹ 

[1] Department of Pharmaco-Biology, Laboratory of Biochemistry and Molecular Biology, University of Bari and CNR Unit for the Study of Mitochondria and Bioenergetics, Via Orabona 4, 70125 Bari, Italy;The M.R.C. Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK

关键词: Yeast mitochondrion;    Transport;    ACR1 gene;    Succinate-fumarate exchange;   

DOI  :  10.1016/S0014-5793(97)01269-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The protein encoded by the ACR1 gene in Saccharomyces cerevisiae belongs to a family of 35 related membrane proteins that are encoded in the fungal genome. Some of them are known to transport various substrates and products across the inner membranes of mitochondria, but the functions of 28 members of the family are unknown. The yeast ACR1 gene was introduced into Escherichia coli on an expression plasmid. The protein was over-produced as inclusion bodies, which were purified and solubilised in the presence of sarkosyl. The solubilised protein was reconstituted into liposomes and shown to transport fumarate and succinate. Its physiological role in S. cerevisiae is probably to transport cytoplasmic succinate, derived from isocitrate by the action of isocitrate lyase in the cytosol, into the mitochondrial matrix in exchange for fumarate. This exchange activity and the subsequent conversion of fumarate to oxaloacetate in the cytosol would be essential for the growth of S. cerevisiae on ethanol or acetate as the sole carbon source.

【 授权许可】

Unknown