Multiple regulatory elements control the expression of the yeast ACR1 gene

FEBS Letters

Valdes, Eva¹  Rodicio, Rosaura¹  Redruello, Begoña¹  Luz Lopez, M.¹ 

[1] Departamento de Bioquı́mica y Biologı́a Molecular, Facultad de Medicina, Universidad de Oviedo, 33006 Oviedo, Spain

关键词: ACR1 gene;    Succinate-fumarate transporter;    Ethanol metabolism;    Carbon source response element;    Stress response promoter element;   

DOI  :  10.1016/S0014-5793(99)00120-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ACR1 gene, encoding a succinate-fumarate transporter, is required by the yeast Saccharomyces cerevisiae for ethanol utilization. Accordingly, gene expression is induced by ethanol and repressed by glucose. Here, we investigated three carbon source response elements present in its promoter region. Specific deletions as well as functional analysis of the elements in a heterologous promoter confirmed their role in transcriptional regulation. Protein binding to carbon source response elements of the ICL1 promoter was competed by all three elements to various extents by the respective ACR1 sequences. In addition, two putative stress response promoter elements present in the ACR1 promoter were investigated in deletion analyses and shown to contribute to gene expression.

【 授权许可】

Unknown