Involvement of integrins and the cytoskeleton in modulation of skeletal muscle glycogen synthesis by changes in cell volume

FEBS Letters

Rennie, Michael J¹  Low, Sylvia Y¹  Taylor, Peter M¹ 

[1] Department of Anatomy and Physiology, University of Dundee, Dundee DD1 4HN, UK

关键词: Integrin;    Glycogen synthesis;    Cell volume;    Skeletal muscle;    Cytoskeleton;    MAP kinase;    mitogen-activated protein kinase;    PI3-kinase;    phosphatidylinositol 3-kinase;    FAK;    focal adhesion kinase;   

DOI  :  10.1016/S0014-5793(97)01264-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Muscle glycogen synthesis is modulated by physiologically relevant changes in cell volume. We have investigated the possible involvement of integrin-extracellular matrix interactions in this process using primary cultures of rat skeletal muscle subject to hypo- or hyper-osmotic exposure with integrin binding peptide GRGDTP to disrupt integrin actions and the inactive analogue GRGESP as control. Osmotically induced increases (77%) and decreases (34%) in glycogen synthesis (d-[¹⁴C]-glucose incorporation into glycogen) were prevented by GRGDTP (but not GRGESP) without affecting glucose transport. Cytoskeletal disruption with cytochalasin D or colchicine had similar effects to GRGDTP. Osmotically induced modulation of muscle glycogen synthesis involves integrin-extracellular matrix interactions and cytoskeletal elements, possibly as components of a cell-volume `sensing' mechanism.

【 授权许可】

Unknown