Nitrogen source‐dependent expression of a 126 kDa protein in the plasma membrane of the cyanobacterium Synechococcus PCC 7942

FEBS Letters

Zinovieva, Maria¹  Fresneau, Chantal¹  Arrio, Bernard¹ 

[1] Laboratoire de Bioénergétique Membranaire, C.N.R.S., Bâtiment 433, Université de Paris-XI, 91405 Orsay Cedex, France

关键词: Nitrogen source;    Plasma membrane protein;    Adaptation;    Nitrate transport;    Synechococcus;    MSX;    l-methionine-d l-sulfoximine;    TCA;    trichloroacetic acid;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;    TDAB-PAGE;    tetradecyltrimethylammonium bromide-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/S0014-5793(97)01199-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The expression of a 126 kDa protein in the cytoplasmic membrane of Synechococcus PCC 7942 is shown to be dependent on the nitrogen source. It is absent in ammonium-grown cells and its quantity is inversely related to the concentration of nitrate or nitrite in the growth medium. Addition of ammonium-grown cells to a medium containing nitrate or l-methionine-d l-sulfoximine results in the expression of this protein. It is present in the plasmalemma of the Synechococcus NC3 mutant (nrtC gene deleted) and absent in the NA3 mutant (nrtABCD genes deleted). These results may suggest involvement of the 126 kDa protein in nitrate transport through Synechococcus cytoplasmic membrane.

【 授权许可】

Unknown