NTR1 encodes a high affinity oligopeptide transporter in Arabidopsis

FEBS Letters

Rouhara, Ila¹  Laloi, Maryse³  Frommer, Wolf B.³  Delrot, Serge²  Schmelzer, Elmon¹  Rentsch, Doris³ 

[1] MPI für Züchtungsforschung, Carl von Linné Weg 10, D-50829 Köln, Germany;CNRS, Université de Poitiers, 25 Rue du Faubourg Saint-Cyprien, 86000 Poitiers, France;Institut für Genbiologische Forschung, Ihnestraβe 63, D-14195 Berlin, Germany

关键词: Amino acid transport;    Nitrate transport;    Peptide transport;    Plant;    Plasma membrane;    Yeast;    CCCP;    carbonyl cyanide m-chlorophenylhydrazone;    DEPC;    diethylpyrocarbonate;    2;    4-DNP;    2;    4-dinitrophenol;    pCMBS;    p-chloromercuribezenesulfonic acid;    NEM;    N-ethylmaleimide;   

DOI  :  10.1016/0014-5793(95)00853-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Heterologous complementation of yeast mutants has enabled the isolation of genes encoding several families of amino acid transporters. Among them, NTR1 codes for a membrane protein with weak histidine transport activity. However at the sequence level, NTR1 is related to rather non-specific oligopeptide transporters from a variety of species including Arabidopsis and to the Arabidopsis nitrate transporter CHL1. A yeast mutant deficient in oligopeptide transport was constructed allowing to show that NTR1 functions as a high affinity, low specificity peptide transporter. In siliques NTR1-expression is restricted to the embryo, implicating a role in the nourishment of the developing seed.

【 授权许可】

Unknown