Inhibition of glucose‐induced insulin secretion by long‐term preexposure of pancreatic islets to leptin

FEBS Letters

Roduit, Raphaël¹  Thorens, Bernard¹ 

[1] Institute of Pharmacology and Toxicology, University of Lausanne, 27, Rue du Bugnon, CH-1005 Lausanne, Switzerland

关键词: Insulin secretion;    Leptin receptor;    Obesity;    Pancreatic islet;   

DOI  :  10.1016/S0014-5793(97)01115-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Here we evaluated the effect of leptin on glucose-induced insulin secretion by normal rat pancreatic islets. We show in perifusion experiments that leptin had no acute effect on the secretory activity of β-cells. However, following preexposure to leptin a pronounced time- and dose-dependent inhibition of both first and second phases of secretion was observed. Maximum inhibition was obtained at 24 h and with 100 nM leptin. This inhibition did not involve a decrease in cellular insulin content. It was also not observed with islets from fa/fa rats. Leptin thus inhibits insulin secretion by a mechanism which requires long-term preexposure to the hormone and which may involve alteration in β-cell gene expression.

【 授权许可】

Unknown