FEBS Letters | |
Inhibition of glucose‐induced insulin secretion by long‐term preexposure of pancreatic islets to leptin | |
Roduit, Raphaël1  Thorens, Bernard1  | |
[1] Institute of Pharmacology and Toxicology, University of Lausanne, 27, Rue du Bugnon, CH-1005 Lausanne, Switzerland | |
关键词: Insulin secretion; Leptin receptor; Obesity; Pancreatic islet; | |
DOI : 10.1016/S0014-5793(97)01115-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Here we evaluated the effect of leptin on glucose-induced insulin secretion by normal rat pancreatic islets. We show in perifusion experiments that leptin had no acute effect on the secretory activity of β-cells. However, following preexposure to leptin a pronounced time- and dose-dependent inhibition of both first and second phases of secretion was observed. Maximum inhibition was obtained at 24 h and with 100 nM leptin. This inhibition did not involve a decrease in cellular insulin content. It was also not observed with islets from fa/fa rats. Leptin thus inhibits insulin secretion by a mechanism which requires long-term preexposure to the hormone and which may involve alteration in β-cell gene expression.
【 授权许可】
Unknown
【 预 览 】
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