Interleukin‐1 induces rapid and transient expression of the c‐fos proto‐oncogene in isolated pancreatic islets and in purified β‐cells

FEBS Letters

Watson, Mark A.¹  Turk, John¹  McDaniel, Michael L.¹  Hughes, Jonathan H.¹  Easom, Richard A.¹ 

[1] Department of Pathology, Washington University School of Medicine, Saint Louis, MO 63110, USA

关键词: Interleukin 1;    Pancreatic islet;    β-cell;    c-fos;    Insulin secretion;   

DOI  :  10.1016/0014-5793(90)81499-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of interleukin-1β (IL-1) on expression of c-fos mRNA in isolated rat pancreatic islets was examined. Accumulation of c-fos mRNA was demonstrable after 30 min of exposure to IL-1, peaked by 60 min, and declined thereafter. Fluorescence-activated cell sorting (FACS) of dispersed islet cells was employed to localize the accumulation of c-fos mRNA to the β-cell. Cycloheximide did not influence the induction of c-fos mRNA by IL-1. Accumulation of c-fos mRNA therefore appears to be an early signal transduction event in the β-cell and a component of the cellular mechanism(s) by which IL-1 influences β-cell function.

【 授权许可】

Unknown