Selection and identification of single domain antibody fragments from camel heavy‐chain antibodies

FEBS Letters

Arbabi Ghahroudi, M¹  Muyldermans, S¹  Hamers, R¹  Desmyter, A¹  Wyns, L¹ 

[1] Vlaams Interuniversitair Instituut voor Biotechnologie, Vrije Universiteit Brussel, Paardenstraat 65, B-1640 Sint Genesius Rode, Belgium

关键词: Antibody;    Phage display;    Single domain antibody fragment;    VH;    Camel;    CDR;    complementarity determining region;    CRAbs;    chelating recombinant antibodies;    Fab;    antigen binding fragment of an antibody;    Fv;    heterodimer of VH and VL;    PCR;    polymerase chain reaction;    SDS-PAGE;    sodium dodecyl sulphate-polyacrylamide gel electrophoresis;    VH;    heavy-chain variable domain;    VHH;    variable domain of heavy-chain antibodies;    VL;    light-chain variable domain. Substitutions are denoted by the wild type amino acid followed by the residue number and the new amino acid;   

DOI  :  10.1016/S0014-5793(97)01062-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Functional heavy-chain γ-immunoglobulins lacking light chains occur naturally in Camelidae. We now show the feasibility of immunising a dromedary, cloning the repertoire of the variable domains of its heavy-chain antibodies and panning, leading to the successful identification of minimum sized antigen binders. The recombinant binders are expressed well in E. coli, extremely stable, highly soluble, and react specifically and with high affinity to the antigens. This approach can be viewed as a general route to obtain small binders with favourable characteristics and valuable perspectives as modular building blocks to manufacture multispecific or multifunctional chimaeric proteins.

【 授权许可】

Unknown