Co‐selection of cognate antibody‐antigen pairs by selectively‐infective phages

FEBS Letters

Desplancq, Dominique¹  Krebber, Claus¹  Plückthun, Andreas¹  Spada, Stefania¹ 

[1] Biochemisches Institut, Universität Zürich, Winterthurerstr. 190, CH-8057 Zürich, Switzerland

关键词: Selectively-infective phage;    Phage display;    Antibody engineering;    Filamentous phage;    Single-chain Fv fragment;    aa;    amino acid;    amp;    ampicillin;    cam;    chloramphenicol;    cfu;    colony forming units;    ELISA;    enzyme linked immunosorbent assay;    HAG;    hemagglutinin;    hag;    peptide from hemagglutinin bound by antibody 17/9;    kan;    kanamycin;    OD550;    optical density at 550 nm;    PBS;    phosphate-buffered saline;    PCR;    polymerase chain reaction;    pfu;    plaque forming units;    scFv;    single-chain Fv fragment;    SIP;    selectively-infective phage;    tet;    tetracycline;    VH;    variable domain of the heavy chain;    VL;    variable domain of the light chain;    wt;    wild type;   

DOI  :  10.1016/0014-5793(95)01348-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have developed a chloramphenicol resistant derivative of fd phage with which cognate pairs of antibodies and antigens can be selected. The phage genome encodes a fusion of single-chain antibody to the C-terminal domain of gIIIp, rendering the phage non-infective. The antigen fused to the N-terminal domains of gIIIp is encoded in the same phage genome. Antigen and antibody fusion interact with each other in the periplasm of the phage-producing cell, restoring infectivity. This system has a very low background and will allow simultaneous randomisation of antibody and antigen.

【 授权许可】

Unknown