Use of phage display for isolation and characterization of single‐chain variable fragments against dihydroflavonol 4‐reductase from Petunia hybrida

FEBS Letters

Depicker, Ann²  De Neve, Myriam²  De Wilde, Chris²  Fischer, Rainer¹  Buys, Emmanuel²  De Jaeger, Geert²  Gerats, Tom²  Van Montagu, Marc²  Bruyns, Anne-Marie²  Eeckhout, Dominique² 

[1] Institut für Biologie I, RWTH Aachen, Worringer Weg 1, D-52074 Aachen, Germany;Laboratorium voor Genetica, Departement Genetica, Vlaams Interuniversitair Instituut voor Biotechnologie (VIB), Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium

关键词: Dihydroflavonol 4-reductase;    Immunomodulation;    Phage display;    Recombinant antibody;    Single-chain variable fragment;    Petunia hybrida;    BSA;    bovine serum albumin;    cfu;    colony-forming units;    CH1;    constant region 1 of heavy chain;    CL;    constant region of light chain;    DFR;    dihydroflavonol 4-reductase;    DFRrec;    recombinant DFR;    ELISA;    enzyme-linked immunosorbent assay;    FU;    fluorescence units;    Ig;    immunoglobulin;    PAGE;    polyacrylamide gel electrophoresis;    PBS;    phosphate-buffered saline;    PCR;    polymerase chain reaction;    scFv;    single-chain variable fragment;    SDS;    sodium dodecyl sulfate;    SOE;    splice-overlap extension;    V;    variable domain;    VH;    variable region of heavy chain;    VL;    variable region of light chain;    Vκ;    variable region of κ light chain;    Vλ;    variable region of λ light chain;    VG1;    variable region of IgG1 heavy chain;    VG2;    variable region of IgG2a/b heavy chain;   

DOI  :  10.1016/S0014-5793(97)00025-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

© 1997 Federation of European Biochemical Societies.

【 授权许可】

Unknown