Cloning and characterization of a Drosophila adenylyl cyclase homologous to mammalian type IX

FEBS Letters

Levin, Lonny R.¹  Kliot, Benjamin¹  Cann, Martin J.¹  Iourgenko, Vadim¹ 

[1] Department of Pharmacology, Cornell University Medical College, 1300 York Avenue, New York, NY 10021, USA

关键词: Drosophila;    Adenylyl cyclase;    cAMP;    Forskolin;    G protein;    Signal transduction;   

DOI  :  10.1016/S0014-5793(97)00891-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel Drosophila adenylyl cyclase (AC) was identified by PCR using degenerate primers specific for the known metazoan ACs. The full-length cDNA predicts a protein displaying significant sequence homology with mammalian Type IX AC (AC9). The abundance and size of the message for the Drosophila AC9 homolog (DAC9) changes through development. Biochemical analysis of DAC9 confirms it encodes a functional enzyme which can be activated by forskolin or G protein. Together with the Drosophila Type I AC homolog encoded by the learning and memory gene, rutabaga, the molecular identification of DAC9 demonstrates there is a family of Drosophila AC isoforms reflecting at least part of the diversity of mammalian AC isoforms.

【 授权许可】

Unknown