Inhibitors of poly(ADP‐ribose) polymerase suppress nuclear fragmentation and apoptotic‐body formation during apoptosis in HL‐60 cells

FEBS Letters

Shiokawa, Daisuke¹  Maruta, Hideharu¹  Tanuma, Sei-ichi¹ 

[1] Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo, Ichigaya, Shinjuku-ku, Tokyo 162, Japan

关键词: Apoptosis;    Poly(ADP-ribosyl)ation;    Cell morphology;    DNA fragmentation;    HL-60 cell;   

DOI  :  10.1016/S0014-5793(97)00887-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of 3-aminobenzamide (3ABm) and benzamide (BAm), known specific inhibitors of poly(ADP-ribose) polymerase (PARP), on actinomycin D (Act D)-induced apoptosis in HL-60 cells were examined. These inhibitors had no appreciable effect on apoptotic DNA fragmentation, chromatin condensation or PARP restriction cleavage, but clearly inhibited morphological changes, especially nuclear fragmentation and apoptotic-body formation, in a dose-dependent manner. These results suggest that the synthesis of ADP-ribose polymers is not essential for the progression of apoptotic DNA fragmentation and chromatin condensation, but is required in the processes leading to nuclear fragmentation and the subsequent apoptotic-body formation during apoptosis in HL-60 cells.

【 授权许可】

Unknown