PP2Cγ: a human protein phosphatase with a unique acidic domain

FEBS Letters

Travis, Sue M¹  Welsh, Michael J¹ 

[1] Department of Internal Medicine, 500 EMRB, University of Iowa College of Medicine, Iowa City, IA 52242, USA

关键词: Protein phosphatase 2C;    Protein phosphorylation;    acidic domain;    cDNA;    RACE;    rapid amplification of cDNA ends;    PCR;    polymerase chain reaction;    bp;    basepair(s);    kb;    kilobase(s);   

DOI  :  10.1016/S0014-5793(97)00837-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have cloned a novel cDNA from human skeletal muscle which encodes a protein phosphatase with a unique acidic domain. It is 34% identical to mammalian PP2Cα and PP2Cβ, and we call it PP2Cγ. It more closely resembles PP2Cs from Paramecium tetraurelia and Schizosaccharomyces pombe than mammalian PP2Cs. Northern blot analysis shows that PP2Cγ is widely expressed, and is most abundant in testis, skeletal muscle, and heart. Like known PP2Cs, recombinant PP2Cγ requires Mg²⁺ or Mn²⁺ for activity. Unlike any other known phosphatase, PP2Cγ has a highly acidic domain: 75% of the 54 residues are glutamate or aspartate.

【 授权许可】

Unknown