The cloning expression and tissue distribution of human PP2Cβ

FEBS Letters

Marley, Anna E.¹  Sullivan, Jane E.¹  Kline, Adam¹  Beri, Raj K.¹  Crabtree, Garry¹ 

[1] Cardiovascular, Musculoskeletal and Metabolism Department, Zeneca Pharmaceuticals, Alderley Park, Macclesfield, Cheshire SK10 4TG, UK

关键词: Protein phosphatase 2C;    Protein phosphorylation;    PCR;    polymerase chain reaction;    bp;    base pair(s);    kb;    kilobase(s);   

DOI  :  10.1016/S0014-5793(98)00708-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have cloned a novel PP2Cβ isoform from a human liver cDNA library which codes for a protein homologous to other mammalian PP2Cβs at the N-terminus but with an extended C-terminus that is unique amongst the PP2Cs. The protein expressed in E. coli is indistinguishable from human recombinant PP2Cα in its cation dependence and insensitivity to okadaic acid. Northern blot analysis of PP2Cβ along with that of PP2Cα shows that human PP2Cs are widely expressed and are most abundant in heart and skeletal muscle.

【 授权许可】

Unknown