Mutational analysis of the mouse 5‐HT7 receptor: importance of the third intracellular loop for receptor–G‐protein interaction

FEBS Letters

King, Linda A²  Beadle, David J²  Bermudez, Isabel²  Obosi, Louis A²  Hen, René¹ 

[1] Center for Neurobiology and Behaviour, Columbia Unversity, New York, USA;School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane Campus, Oxford OX3 0BP, UK

关键词: 5-HT7 receptor;    G-protein;    Mutagenesis;    Baculovirus expression;   

DOI  :  10.1016/S0014-5793(97)00813-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The mouse serotonin (5-HT) receptor subtype, 5-HT₇, belongs to the family of seven transmembrane G-protein-coupled receptors. To identify the structural basis for the coupling of 5-HT₇ receptor to Gα_S we constructed a number of receptor mutants in which amino acid residues were either substituted or deleted from the second and third intracellular loops. Wild-type and mutant 5-HT₇ receptors were expressed in insect cells using the baculovirus vectors. Two mutant receptor species, 5-HT₇(E325G) and 5-HT₇(K327S), demonstrated markedly impaired abilities to stimulate adenylyl cyclase. The results suggest the importance of the C-terminal region of the third intracellular loop in receptor–G-protein interaction and that specific charged residues, E325 and K327, may play a critical role in this interaction.

【 授权许可】

Unknown