Functional characterisation of the Drosophila 5‐HT dro1 and 5‐HT dro2Bserotonin receptors in insect cells: activation of a Gαs ‐like protein by 5‐HT dro1 but lack of coupling to inhibitory G‐proteins by 5‐HT dro2B

FEBS Letters

Europe-Finner, G.Nicholas¹  King, Linda A.³  Obosi, Louis A.³  Schuette, Diana G.³  Beadle, David J.³  Bermudez, Isabel³  Hen, René² 

[1] University of Oxford, Nuffield Department of Obstetrics and Gynecology, John Radcliffe Hospital, Oxford, UK;Center for Neurobiology and Behaviour, Columbia University, New York, USA;School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane Campus, Oxford OX3 0BP, UK

关键词: Drosophila 5-HT dro1receptor;    Drosophila 5-HT dro2Breceptor;    Baculovirus expression;    Insect cell (Sf9);    G-protein;   

DOI  :  10.1016/0014-5793(96)00078-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Insect cells are routinely used for the production of receptor proteins. Expression of the Drosophila 5-HT _dro1 serotonin receptor resulted in positive coupling of the receptor to adenylyl cyclase via the G_αs G-protein subtype. The Drosophila 5-HT _dro2B receptor stimulated the metabolism of inositol phospholipid via a pertussis toxin-insensitive G-protein, but exhibited no detectable inhibition of adenylyl cyclase. Immunoblot analysis of the endogenous G-proteins revealed that Sf9 cells lack the G-protein subtypes G_αi1–3 and G_α0, but express the subtypes G_αs and G_αq .

【 授权许可】

Unknown