High‐level expression of functional cGMP‐dependent protein kinase using the baculovirus system

FEBS Letters

Müller, Stefan²  Feil, Robert¹  Hofmann, Franz¹ 

[1] Institut für Pharmakologie und Toxikologie der Technischen Universität München, Biedersteiner Str. 29, 80802 München, Germany;Genzentrum, MPI für Biochemie, 82152 Martinsried, Germany

关键词: cGMP-dependent protein kinase;    Baculovirus expression;   

DOI  :  10.1016/0014-5793(93)81632-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The understanding of the structure and function of cGMP-dependent protein kinase (cGMP kinase) has been hindered by the difficulty to obtain large quantities of functional enzyme. A recombinant baculovirus encoding bovine cGMP kinase la was constructed and purified. Infected insect cells synthesized large amounts of soluble and biologically active cGMP kinase Iα representing up to 10% of the total cell extract protein. The recombinant enzyme had an identical apparent molecular mass, cGMP affinity and kinase activity as the native bovine lung enzyme. The high-level expression of functional cGMP kinase Iα should provide an excellent tool to study further the structure and function of cGMP kinase.

【 授权许可】

Unknown