Phosphocholine and sphingosine‐1‐phosphate synergistically stimulate DNA synthesis by a MAP kinase‐dependent mechanism

FEBS Letters

Kiss, Zoltan¹  Mukherjee, Jagat J¹ 

[1] The Hormel Institute, University of Minnesota, 801 16th Avenue NE, Austin, MN 55912, USA

关键词: Phosphocholine;    Sphingosine-1-phosphate;    MAP kinase;    DNA synthesis;    PCho;    phosphocholine;    S1P;    sphingosine-1-phosphate;    MAP;    mitogen-activated protein;   

DOI  :  10.1016/S0014-5793(97)00776-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have previously shown that in NIH 3T3 fibroblasts phosphocholine (PCho) potentiates sphingosine-1-phosphate (S1P)-induced mitogenesis. Here we report that PCho and S1P also synergistically stimulate DNA synthesis in mouse Swiss 3T3 fibroblasts and in mouse JB6 epidermal cells. The combined actions of PCho and S1P on DNA synthesis were associated with synergistic activation of the p42/p44 mitogen-activated protein (MAP) kinases. Ethanolamine (50–100 μM) further enhanced the synergistic effects of PCho and S1P on DNA synthesis but not on MAP kinase activity. The results indicate that the synergistic mitogenic effects of PCho and S1P (i) are not restricted to NIH 3T3 fibroblasts, (ii) are predominantly mediated by the MAP kinase-dependent signal transduction pathway, and (iii) are enhanced by ethanolamine via a MAP kinase-independent mechanism.

【 授权许可】

Unknown