Thyroxine induces cyclosporin A‐insensitive, Ca2+‐dependent reversible permeability transition pore in rat liver mitochondria

FEBS Letters

Simonian, R.A¹  Malkevitch, N.V¹  Skulachev, V.P¹  Dedukhova, V.I¹  Starkov, A.A¹ 

[1]Department of Bioenergetics, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, 119899 Moscow, Russia

关键词: Permeability transition pore;    Cyclosporin A;    Thyroxine;    Mitochondria;    ΔΨ;    transmembrane electric potential difference;    BSA;    bovine serum albumin;    DNP;    2;    4-dinitrophenol;    EGTA;    ethylene glycol-bis(β-aminoethylether)-N;    N;    N′;    N′-tetraacetic acid;    MOPS;    morpholinopropane sulfonate;    T4;    l-thyroxine;    CS;    cyclosporin A;    DTE;    dithioerythritol;    ANT;    adenine nucleotide translocase;   

DOI  :  10.1016/S0014-5793(97)00666-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of thyroxine on Ca²⁺-dependent mitochondrial permeability transition has been examined. It is shown that 40 μM thyroxine induces high amplitude swelling and decrease in membrane potential in Ca²⁺-loaded rat liver mitochondria, both in the presence and absence of cyclosporin A. Thyroxine-induced decrease in membrane potential is partially or completely reversed by addition of EGTA into the incubation medium. Nigericin and ADP are shown to prevent, or significantly delay, the effects of thyroxine on both mitochondrial swelling and membrane potential, whereas nicotinamide potentiates the permeabilisation of mitochondria. It is suggested that thyroxine induced reversible, cyclosporin A-insensitive permeability transition pore (PTP) opening in the inner mitochondrial membrane.

【 授权许可】

Unknown