Cyclosporin A‐sensitive release of Ca2+ from mitochondria in intact thymocytes

FEBS Letters

Chernyak, Boris V.¹ 

[1] A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russia

关键词: Thymocyte;    Mitochondrion;    Ca2+;    Cyclosporin A;    Permeability transition pore;    PTP;    permeability transition pore;    CsA;    cyclosporin A;    TG;    thapsigargin;    PhAsO;    phenylarsine oxide;    FCCP;    carbonylcyanide-p-trifluoromethoxyphenyl hydrazone;    t-BOOH;    tert-butylhydroperoxide;    6-Kch;    6-ketocholestanol;   

DOI  :  10.1016/S0014-5793(97)01361-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Release of Ca²⁺ from mitochondria into cytosol in intact thymocytes was studied using the fluorescent dye Fluo-3. It was shown that the release of Ca²⁺ induced by the dithiol cross-linking agent phenylarsine oxide or by uncoupler was strongly inhibited by cyclosporin A, a specific inhibitor of the permeability transition pore (PTP) in mitochondria. Oxidative stress sensitized the pore so even partial uncoupling caused rapid cyclosporin A-sensitive release of Ca²⁺. The experiments on digitonin-permeabilized cells confirmed that uncoupling induced opening of the PTP, which forms the major pathway for rapid release of Ca²⁺ from thymocyte mitochondria.

【 授权许可】

Unknown