Cell cycle specificity of Fas‐mediated apoptosis in WIL‐2 cells

FEBS Letters

Nikonova, Larisa V²  Beletskaya, Irina V¹  Beletsky, Igor P² 

[1] Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Science, 142292 Puschino, Moscow region, Russian Federation;Institute of Theoretical and Experimental Biophysics, Russian Academy of Science, 142292 Puschino, Moscow region, Russian Federation

关键词: Fas/APO1-mediated apoptosis;    Cell cycle;    p53 antigen;   

DOI  :  10.1016/S0014-5793(97)00740-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Antibodies to Fas/APO1 receptor induce effective apoptosis in WIL-2 cells of the human B-lymphoid line. Quantitative assessment of the extent of the death in cells synchronized by thymidine block revealed a significant increase in their sensitivity to the cytocidal effect mediated by Fas/APO1 during the G₁ phase of the cell cycle. Western analysis of the content of the p53 antigen in the cytoplasm and nuclei of the cells showed that the Fas/APO1-induced death is accompanied by massive translocation of the p53 from the cytoplasm to the nucleus. These findings suggest that cell vulnerability to the Fas/APO1-mediated apoptosis is subjected to regulation by cell cycle-dependent mechanisms, one of which is probably the function of the p53 antigen.

【 授权许可】

Unknown