Further studies on the recoupling effect of 6‐ketocholestanol upon oxidative phosphorylation in uncoupled liver mitochondria

FEBS Letters

Chávez, Edmundo¹  Cuéllar, Adela¹  Ramı́rez, Jorge²  Infante, Victor M.¹ 

[1] Departamento de Bioquı́mica, Instituto Nacional de Cardiologı́a Ignacio Chávez, Juan Badiano 1, México D.F. 014080, Mexico;Departamento de Microbiologı́a, Instituto de Fisiologı́a Celular UNAM, México D.F. 014080, Mexico

关键词: Liver mitochondria;    Submitochondrial particle;    6-Ketocholestanol;    Uncoupling;    Cytochrome-oxidase-proteoliposome;    6-kch;    6-ketocholestanol (5α-cholestan-3β-ol-6-one);    Δψ;    transmembrane electrical potential difference;    CCCP;    carbonylcyanide-3-chlorophenylhydrazone;    FCCP;    carbonylcyanide p-trifluoromethoxyphenylhydrazone;    DPH;    1;    6-diphenyl-1;    3;    5-hexatrieno;    SMP;    submitochondrial particles;    TMPD;    N;    N;    N′;    N′-tetramethyl-p-phenylendiamine dihydrochloride;   

DOI  :  10.1016/S0014-5793(97)00741-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of 6-ketocholestanol was studied on CCCP-induced uncoupling in liver mitochondria, submitochondrial particles and cytochrome oxidase proteoliposomes. It was found that 6-ketocholestanol prevents and reverses uncoupling induced by nM concentrations of CCCP on the three systems assayed. As it was reported on kidney mitochondrial membranes [Chávez et al. (1996) FEBS Lett. 379, 305–308], the recoupling effect caused by 6-ketocholestanol on submitochondrial particles and proteoliposomes could be due to a diminution of membrane fluidity.

【 授权许可】

Unknown