On the mechanism by which 6‐ketocholestanol protects mitochondria against uncoupling‐induced Ca2+ efflux

FEBS Letters

Chávez, Edmundo²  Rodríguez-Enríquez, Sara²  Moreno-Sánchez, Rafael²  Ramírez, Jorge¹  Cuéllar, Adela²  Bravo, Concepción²  Zazueta, Cecilia²  Reyes-Vivas, Horacio² 

[1] Departamento de Microbiología, Instituto de Fisiología Celular, UNAM, México, DF 014080, México;Departamento de Bioquímica, Instituto Nacional de Cardiología, Ignacio Chávez, Juan Badiano No. 1, México, DF 014080, México

关键词: Mitochondrial Ca2+;    Calcium;    Mitochondrion (kidney);    Permeability transition;    6-Ketocholestanol;    Mitochondrial membrane;    kCh;    6-ketocholestanol;    CCP;    carbonyl cyanide-m-chlorophenyl hydrazone;    CAT;    carboxyatractyloside;    Pi;    inorganic phosphate;    MD;    menadione;    DPH;    1;    6-diphenyl-1;    3;    5-hexatriene;    ANS;    8-aniline-naphthalene sulfonic acid;   

DOI  :  10.1016/0014-5793(95)01533-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

This work shows that 6-ketocholestanol (kCh) inhibits the effect of carbonyl cyanide-m-chlorophenyl hydrazone (CCP) on mitochondrial Ca²⁺ efflux. Such an effect proved to be caused by diminution of membrane fluidity, therefore, it is affected by the incubation temperature. Furthermore, kCh reversed CCP-induced Ca²⁺ efflux depending on the accumulation of phosphate. It is also shown that kCh enhances the effect of carboxyatractyloside on membrane permeability transition.

【 授权许可】

Unknown