Specificity of different isoforms of protein phosphatase‐2A and protein phosphatase‐2C studied using site‐directed mutagenesis of HMG‐CoA reductase

FEBS Letters

Grahame Hardie, D²  Tamura, Shinri¹  Kobayashi, Takayasu¹  Pang Ching, Yick² 

[1]Department of Biochemistry, Division of Gene Research, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, Sendai 980, Japan

[2]Biochemistry Department, The University, Dundee, DD1 4HN, Scotland, UK

关键词: Protein phosphatase-2A;    Protein phosphatase-2C;    dephosphorylation;    HMG-CoA reductase;    AMP-activated protein kinase;    Site-directed mutagenesis;    AMPK;    AMP-activated protein kinase;    HMG-CoA;    3-hydroxy-3-methylglutaryl-coenzyme A;    PP1;    protein phosphatase-1;    PP2AC;    protein phosphatase-2A catalytic subunit;    PP2A1;    protein phosphatase-2A ABC heterotrimer;    PP2C;    protein phosphatase-2C;    PBS;    phosphate-buffered saline;    Sp;    phosphoserine;    Tp;    phosphothreonine;   

DOI  :  10.1016/S0014-5793(97)00712-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have expressed the catalytic domain of Chinese hamster HMG-CoA reductase, and 13 point mutations involving the region around the single phosphorylation site for AMP-activated protein kinase. After phosphorylation, these were used to test the specificity of isoforms of protein phosphatase-2A [bovine PP2A_C (catalytic subunit) and PP2A₁ (ABC heterotrimer)] and protein phosphatase-2C (human α; mouse α, β1, β2, β3, β4, β5). PP2A₁ had >50-fold higher activity for HMG-CoA reductase variants than PP2A_C, but their relative selectivity for different variants was similar. Although the specificities of PP2A and PP2C were distinct, no dramatic differences in selectivity were observed between different PP2C isoforms.

【 授权许可】

Unknown