Distinct type‐2A protein phosphatases activate HMGCoA reductase and acetyl‐CoA carboxylase in liver

FEBS Letters

Hardie, D.Grahame¹  Hue, Louis²  Skarlas, Polymnia²  Ching, Yick Pang¹  Gaussin, Vinciane² 

[1] Biochemistry Department, Dundee University, Dundee, DD1 4HN Scotland, UK;Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology, Louvain University Medical School, Avenue Hippocrate 75, Brussels B-1200, Belgium

关键词: Acetyl-CoA carboxylase;    AMP-activated protein kinase;    Glutamine;    HMGCoA reductase;    Protein phosphatase-2A;    ACC;    acetyl-CoA carboxylase;    AICAriboside;    5-amino 4-imidazolecarboxamide riboside;    AMPK;    AMP-activated protein kinase;    GAPP;    hepatic glutamate-activated protein phosphatase;    HMGCoA;    hydroxymethylglutaryl coenzyme A;    PP;    protein phosphatase;   

DOI  :  10.1016/S0014-5793(97)00890-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Acetyl-CoA carboxylase and HMGCoA reductase are inactivated by the same AMP-activated protein kinase and are activated by type-2A protein phosphatase. To determine whether the same species of protein phosphatase-2A were involved, we studied the interconversion of acetyl-CoA carboxylase and HMGCoA reductase in isolated rat hepatocytes. We show that (i) these enzymes are differently regulated in hepatocytes and (ii) the species of type-2A protein phosphatase involved in their activation are different and can be separated by anion-exchange chromatography.

【 授权许可】

Unknown