Frontiers in Chemistry | |
Spatial sterol metabolism unveiled by stimulated Raman imaging | |
Chemistry | |
Yihui Zhou1  Hyeon Jeong Lee1  Hanlin Zhu2  Yongqing Zhang2  Delong Zhang2  Cunqi Ye3  Wen Fang3  | |
[1] Key Laboratory for Biomedical Engineering of Ministry of Education, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, China;Zhejiang Province Key Laboratory of Quantum Technology and Device, Department of Physics, Interdisciplinary Centre for Quantum Information, Zhejiang University, Hangzhou, China;Zhejiang Provincial Key Laboratory for Cancer Molecular Cell Biology, Life Sciences Institute, Zhejiang University, Hangzhou, China; | |
关键词: stimulated Raman scattering (SRS) microscopy; metabolite imaging; biophysics; cholesterol; genetic engineering; HMGCoA reductase; yeast; | |
DOI : 10.3389/fchem.2023.1166313 | |
received in 2023-02-15, accepted in 2023-03-13, 发布年份 2023 | |
来源: Frontiers | |
【 摘 要 】
Spatiotemporal dynamics of small-molecule metabolites have gained increasing attention for their essential roles in deciphering the fundamental machinery of life. However, subcellular-level regulatory mechanisms remain less studied, particularly due to a lack of tools to track small-molecule metabolites. To address this challenge, we developed high-resolution stimulated Raman scattering (SRS) imaging of a genetically engineered model (GEM) to map metabolites in subcellular resolution. As a result, an unexpected regulatory mechanism of a critical metabolite, sterol, was discovered in yeast by amplifying the strength of vibrational imaging by genetic modulation. Specifically, isozymes of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) were evident to promote ergosterol distribution to distinct subcellular locations, where ergosterol was enriched by a local HMGR-directed synthesis. The heterogeneity of this expression pattern thus provides new insights into sterol metabolism and related disease treatment strategies. These findings demonstrate SRS-GEM as a promising platform for new possibilities in investigating metabolic regulation, disease mechanisms, and biopharmaceutical research.
【 授权许可】
Unknown
Copyright © 2023 Zhang, Zhou, Fang, Zhu, Ye, Zhang and Lee.
【 预 览 】
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