A Mox homeobox gene in the gastropod mollusc Haliotis rufescens is differentially expressed during larval morphogenesis and metamorphosis

FEBS Letters

Morse, Daniel E.¹  Degnan, Bernard M.²  Degnan, Sandie M.²  Fentenany, Gabriel¹ 

[1] Marine Biotechnology Center and Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, CA 93106, USA;Department of Zoology and Centre for Molecular and Cellular Biology, University of Queensland, Brisbane, Qld 4072, Australia

关键词: Homeobox evolution;    Mollusc;    Mox;    Quantitative RT-PCR;    (Haliotis);   

DOI  :  10.1016/S0014-5793(97)00571-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated a homeobox-containing cDNA from the gastropod mollusc Haliotis rufescens that is most similar to members of the Mox homeobox gene class. The derived Haliotis homeodomain sequence is 85% identical to mouse and frog Mox-2 homeodomains and 88.9% identical to the partial cnidarian cnox5-Hm homeodomain. Quantitative reverse transcription-polymerase chain reaction analysis of mRNA accumulation reveals that this gene, called HruMox, is expressed in the larva, but not in the early embryo. Transcripts are most prevalent during larval morphogenesis from trochophore to veliger. There are also transient increases in transcript prevalence 1 and 3 days after the intitiation of metamorphosis from veliger to juvenile. The identification of a molluscan Mox homeobox gene that is more closely related to vertebrate genes than other protostome (e.g. Drosophila) genes suggests the Mox class of homeobox genes may consist of several different families that have been conserved through evolution.

【 授权许可】

Unknown