The uptake of cholesterol at the small‐intestinal brush border membrane is inhibited by apolipoproteins

FEBS Letters

Weber, Franz E³  Boffelli, Dario³  Hauser, Helmut³  Werder, Moritz³  Compassi, Sabina³  Phillips, Michael C²  Schulthess, Georg¹ 

[1] Department für Innere Medizin, Medizinische Poliklinik, Universitätsspital, CH-8091 Zürich, Switzerland;Department of Biochemistry, MCP♦Hahnemann Medical School, Allegheny University of the Health Sciences, Philadelphia, PA 19129, USA;Department of Biochemistry, Swiss Federal Institute of Technology, ETH Zentrum, Universitätstrasse 16, CH-8092 Zürich, Switzerland

关键词: Cholesterol;    Intestinal absorption;    Brush border membrane;    Inhibition;    Apolipoprotein;    Apo;    apolipoprotein;    BBM;    brush border membrane;    BBMV;    brush border membrane vesicle(s);    HDL;    high-density lipoprotein;    HPLC;    high performance liquid chromatography;    PAGE;    polyacrylamide gel electrophoresis;    PC;    phosphatidylcholine;    PVDF;    polyvinylidene difluoride;    SDS;    sodium dodecyl sulfate;    SUV;    small unilamellar vesicle(s);   

DOI  :  10.1016/S0014-5793(97)00658-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The uptake of free and esterified cholesterol at the brush border membrane is protein-mediated. Here we show that this sterol uptake is effectively inhibited by exchangeable serum apolipoproteins. Binding of the apolipoprotein to the brush border membrane mediates the inhibitory effect. Evidence is presented to show that the structural motif responsible for the inhibition is the amphipathic α-helix.

【 授权许可】

Unknown