Expression of trypsin in vascular endothelial cells

FEBS Letters

Miyagi, Yohei¹  Koshikawa, Naohiko³  Miyazaki, Kaoru³  Mizushima, Hiroto³  Yanoma, Shunsuke²  Nagashima, Yoji¹  Yasumitsu, Hidetaro³ 

[1] Department of Pathology, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236, Japan;Clinical Research Institute, Kanagawa Cancer Center, 54-2 Nakao-cho, Asahi-ku, Yokohama 241, Japan;Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, Yokohama 244, Japan

关键词: Vascular endothelial cell;    Trypsin;    Angiogenesis;    Disseminated intravascular coagulation;    bp;    base pair;    DIC;    disseminated intravascular coagulation;    HUVEC;    human umbilical vein endothelial cell(s);    MMP;    matrix metalloproteinase;    RT-PCR;    reverse transcription/polymerase chain reaction;    TPA;    12-O-tetradecanoylphorbol-13-acetate;   

DOI  :  10.1016/S0014-5793(97)00565-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Proteinases produced by vascular endothelial cells are expected to play important roles in many biological processes. Here we report that human vascular endothelial cells express trypsinogen-2 mRNA and its protein product in culture. The trypsinogen production was stimulated by a tumor promoter and associated with cell growth. In situ hybridization analysis showed that the trypsinogen gene was significantly expressed in vascular endothelial cells around gastric tumors and in patients with disseminated intravascular coagulation (DIC). These results suggest the possible roles of endothelial cell-derived trypsin in tumor angiogenesis and abnormal blood coagulation.

【 授权许可】

Unknown