A single mutation in the M‐subunit of Rhodospirillum rubrum confers herbicide resistance

FEBS Letters

Sopp, Gabriele¹  Oettmeier, Walter¹  Rutherford, William A² 

[1] Lehrstuhl Biochemie der Pflanzen, Ruhr-Universität, D-44780 Bochum, Germany;Section de Bioénergetique, Département de Biologie Cellulaire et Moléculaire, CNRS URA 1296, CEA Saclay, F-91191 Gif sur Yvette, France

关键词: Bacterial reaction center;    Amino-thiazole;    Glu M234>Lys;    DAD;    diaminodurene;    DCMU;    3-(3′;    4′-dichlorophenyl)-1;    1-dimethyl-urea;    PPCTH;    2-(1-phenyl)ethylamino-3-propionylamino-4-cyano-thiazole;    RC;    reaction center;   

DOI  :  10.1016/S0014-5793(97)00503-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cells of the photosynthetic bacterium Rhodospirillum rubrum were rendered resistant against the inhibitor 2-(1-phenyl)ethylamino-3-propionylamino-4-cyano-thiazole (PPCTH). Electron transport in reaction centers prepared from one of the mutants (M6) was neither inhibited by PPCTH and other NH-thiazoles nor terbutryn. These inhibitors are known to bind at the Q_B site of the L-subunit. Compared to the wild type, chromatophores from M6 exhibited strongly altered Q_B ⁻Fe²⁺ and Q_A ⁻Fe²⁺ EPR signals. Inhibitor resistance is due to a mutation in the bacterial reaction center M-subunit, where Glu²³⁴ is exchanged against Lys. This is the first example of an inhibitor resistance in the Q_B site caused by a mutation in the M-subunit.

【 授权许可】

Unknown