FEBS Letters | |
Probing the secondary quinone (QB) environment in photosynthetic bacterial reaction centers by light‐induced FTIR difference spectroscopy | |
Nabedryk, E.1  Thibodeau, D.L.1  Breton, J.1  Berthomieu, C.1  | |
[1] DBCM/SBE, C.E.N. Saclay, 91191 Gif-sur-Yvette Cedex, France | |
关键词: Fourier transform infrared spectroscopy; Bacterial reaction center; Photosynthesis; Primary quinone; Secondary quinone; Ubiquinone; FTIR; Fourier transform infrared spectroscopy; QA (QB); primary (secondary) quinone electron acceptor; P; primary electron donor; RC; reaction center; UQ; ubiquinone; DAD; diaminodurene; | |
DOI : 10.1016/0014-5793(91)81014-Y | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The photoreduction of the secondary electron acceptor, QB, has been characterized by light-induced Fourier transform infrared difference spectroscopy of Rb. sphaeroides and Rp. viridis reaction centers. The reaction centers were supplemented with ubiquinone (UQ10 or UQ0). The QB− state was generated either by continuous illumination at very low intensity or by single flash in the presence of redox compounds which rapidly reduce the photooxidized primary electron donor P+. This approach yields spectra free from P and P+ contributions making possible the study of the microenvironment of QB and QB−. Assignments are proposed for the CO vibration of QB− and tentatively for the C = O and C = C vibrations of QB.
【 授权许可】
Unknown
【 预 览 】
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RO201912020295229ZK.pdf | 574KB | download |