Probing the primary quinone environment in photosynthetic bacterial reaction centers by light‐induced FTIR difference spectroscopy

FEBS Letters

Nebedryk, E.²  Thibodeau, D.L.²  Vermèglio, A.¹  Breton, J.²  Mäntele, W.³  Berthomieu, C.² 

[1]DPVE. CEN Cadarache. B.P. no. 1, 13108 St. Paul lez Durance Cedex France

[2]DBMC. CEN. Saclay, 91191 Gif-sur-Yvette Cedex, France

[3]Institut für Biophysik und Strahlenbiologie, University Freiburg, Albertstr. 23 7800 Freiburg, Germany

关键词: Fourier transform infrared spectroscopy;    Bacterial reaction center;    Photosynthesis;    Primary quinone;    Secondary quinone;    FTIR;    Fourier transform Infrared spectroscopy;    QA (QB);    primary (secondary) quinone electron acceptor;    QP;    quinone pool;    P;    Primary electron donor;    HA;    intermediary electron acceptor;    RC;    reaction center;    DAD;    diaminodurene;    PSII;    photosystem II;   

DOI  :  10.1016/0014-5793(91)80129-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Photoreduction of the primary electron acceptor, Q_A, has been characterized by light-induced Fourier transform infrared difference spectroscopy for Rb. spaeroides reaction centers und for Rsp. rubrum and Rp. viridis chromatophores. The samples were treated both with redox compounds, which rapidly reduce the photooxidized primary electron donor P⁺, and with inhibitors of electron transfer from Q_A ⁻ to the secondary quinone Q_B. This approach yields spectra free from P and P⁺ contributions which makes possible the study of the microenvironment of Q_A and Q_A ⁻.

【 授权许可】

Unknown