Endogenously produced peroxynitrite induces the oxidation of mitochondrial and nuclear proteins in immunostimulated macrophages

FEBS Letters

Salzman, Andrew L¹  Szabó, Csaba¹  O'Connor, Michael¹ 

[1] Children's Hospital Medical Center, Division of Critical Care, 3333 Burnet Avenue, Cincinnati, OH 45229, USA

关键词: Inflammation;    Superoxide;    Nitric oxide;    Shock;    Oxidation;    Protein;    Macrophage;    Peroxynitrite;    IFNγ;    interferon-gamma;    LPS;    bacterial lipopolysaccharide;    NO;    nitric oxide;    NOS;    nitric oxide synthase;    iNOS;    inducible NOS;    L-NMA;    NG-methyl-l-arginine;    MnTBAP;    Mn(III)tetrakis(4-benzoic acid)porphyrin;    OD;    optical density;   

DOI  :  10.1016/S0014-5793(97)00466-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Here we investigated the role of endogenous nitric oxide (NO) and peroxynitrite in the process of protein oxidation (as measured by the detection of 2,4-dinitrophenylhydrazine-reactive carbonyls) in immunostimulated macrophages. Immunostimulation of the macrophages by bacterial lipopolysaccharide and gamma-interferon (LPS/IFNγ) resulted in a marked increase in the oxidation of a large number of mitochondrial and nuclear proteins. The inhibitor of NO synthase, N^G-methyl-l-arginine (3 mM), and the cell-permeable superoxide dismutase mimetic Mn(III)tetrakis(4-benzoic acid)porphyrin (300 μM) both reduced the extent of protein oxidation in response to LPS/IFNγ. These results support the view that endogenously produced peroxynitrite induces protein oxidation in the mitochondria and nucleus of immunostimulated macrophages.

【 授权许可】

Unknown