Evaluation of the relative contribution of nitric oxide and peroxynitrite to the suppression of mitochondrial respiration in immunostimulated macrophages using a manganese mesoporphyrin superoxide dismutase mimetic and peroxynitrite scavenger

FEBS Letters

Day, Brian J.¹  Szabó, Csaba²  Salzman, Andrew L.² 

[1] Duke University Medical Center, Department of Medicine, Division of Pulmonary and Critical Care Medicine, Durham, NC 27710, USA;Children's Hospital Medical Center, Division of Critical Care, 3333 Burnet Avenue, Cincinnati, OH 45229, USA

关键词: Peroxynitrite;    Nitric oxide;    Endotoxin;    Shock;    Inflammation;    Nitric oxide synthase;    Superoxide;    Mn(III) porphyrins;    Superoxide dismutase;   

DOI  :  10.1016/0014-5793(96)00087-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Here we report that the cell-permeable superoxide dismutase mimetic Mn(III)tetrakis (4-benzoic acid) porphyrin (MnTBAP) inhibits the oxidation of dihydrorhodamine-123 by peroxynitrite, but does not scavenge nitric oxide (NO). MnTBAP protects against the suppression of mitochondrial respiration in J774 cells exposed to peroxynitrite or to NO donors. MnTBAP and provide additive protective effect against the suppression of respiration in immunostimulated cells. Our data suggest separate contributions of NO and peroxynitrite to the suppression of mitochondrial respiration and support the role of oxidative stress in the expression of the inducible isoform of NO synthase.

【 授权许可】

Unknown