Stimulatory effects of parathyroid hormone and 1,25‐dihydroxyvitamin D3 on insulin‐like growth factor‐binding protein‐5 mRNA expression in osteoblastic UMR‐106 cells: the difference between transient and continuous treatments

FEBS Letters

Nasu, Masamichi¹  Chihara, Kazuo¹  Sugimoto, Toshitsugu¹ 

[1] Third Division, Department of Medicine, Kobe University School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650, Japan

关键词: Parathyroid hormone;    1;    25-Dihydroxyvitamin D3;    Insulin-like growth factor-I;    Insulin-like growth factor-binding protein-5;    Type-1 procollagen;    PTH;    parathyroid hormone;    IGF-I;    insulin-like growth factor-I;    IGFBP-5;    insulin-like growth factor-binding protein-5;    1;    25(OH)2D3;    1;    25-dihydroxyvitamin D3;    dbcAMP;    N6;    O2′-dibutyryl adenosine 3′;    5′-cyclic monophosphate;    PMA;    phorbol 12-myristate 13-acetate;   

DOI  :  10.1016/S0014-5793(97)00484-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The transient treatment with parathyroid hormone (PTH) for 12 h, followed by its removal for 36 h, stimulated insulin-like growth factor-binding protein (IGFBP)-5 mRNA expression more strongly than the continuous treatment for 48 h in osteoblastic UMR-106 cells. The transient but not continuous treatment with A23187 also stimulated it. In contrast, 1,25-dihydroxyvitamin D₃ stimulated it, irrespective of the treatment design. IGFBP-5 stimulated type-1 procollagen mRNA expression. The present study first indicated that the transient treatment with PTH more effectively stimulated IGFBP-5 mRNA expression than its continuous treatment partly via an increase in intracellular calcium and suggested that IGFBP-5 might be involved in the anabolic action of PTH in bone.

【 授权许可】

Unknown