1α,25‐Dihydroxyvitamin D3 increases IGF binding protein‐5 expression in cultured osteoblasts

FEBS Letters

Zapf, J.¹  Gosteli-Peter, M.A.¹  Schläpfer, I.¹  Schmid, C.¹  Froesch, E.R.¹  Hauri, C.¹ 

[1] Division of Endocrinology and Metabolism, Department of Internal Medicine, University Hospital, CH-8091 Zürich, Switzerland

关键词: 1;    25-Dihydroxyvitamin D3;    Parathyroid hormone;    Insulin-like growth factor I;    Insulin-like growth factor binding protein-5;    Insulin-like growth factor binding protein-3;   

DOI  :  10.1016/0014-5793(96)00777-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rat osteoblasts produce insulin-like growth factors (IGFs) and IGF binding proteins (IGFBPs). Expression of IGFBP-5, an IGFBP which stimulates DNA synthesis of osteoblasts, was studied in vitro under the influence of 1α,25-dihydroxyvitamin D₃ (1,25(OH)₂D₃) and parathyroid hormone (PTH). These two calcium-regulating hormones stimulated the expression of IGFBP-5 mRNA in cultured rat osteoblasts at low concentrations (10 pM) and in a dose- and time-dependent manner. Intact IGFBP-5 was not detected in the culture medium, but was found attached to the cell layer. IGFBP-5 may thus direct IGFs to the sites of bone remodeling.

【 授权许可】

Unknown