Nitric oxide protects endothelial cells from tumor necrosis factor‐α‐mediated cytotoxicity: possible involvement of cyclic GMP

FEBS Letters

Polte, Tobias¹  Oberle, Stefanie¹  Schröder, Henning¹ 

[1] Department of Pharmacology and Toxicology, School of Pharmacy, Martin Luther University, Wolfgang-Langenbeck-Str. 4, 06099 Halle (Saale), Germany

关键词: Nitric oxide;    Endothelial cell;    Cyclic GMP;    Cytoprotection;    Tumor necrosis factor-α;    NO;    nitric oxide;    ODQ;    1H-[1;    2;    4]oxadiazole[4;    3-a]quinoxalin-1-one;    PTIO;    2-Phenyl-4;    4;    5;    5;    -tetramethylimidazoline-1-oxyl-3-oxide;    Sper/NO;    spermine NONOate;    TNF-α;    tumor necrosis factor-α;   

DOI  :  10.1016/S0014-5793(97)00480-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In cultured endothelial cells, incubation with TNF-α (50 ng/ml) for 48 h markedly reduced viability of endothelial cells. A 6 h preincubation with Sper/NO (0.03-−1 μM) protected endothelial cells in a concentration-dependent manner and increased viability by 63% of control. The NO scavenger PTIO (30 μM) completely abolished cytoprotection by Sper/NO. A cytoprotective effect comparable to Sper/NO was observed when preincubating the cells with 8-bromo cyclic GMP (1–10 μM). Moreover, no protection by Sper/NO occurred in the presence of ODQ (0.1 μM), a selective inhibitor of soluble guanylyl cyclase. Our results demonstrate that NO produces a long-term endothelial protection against cellular injury by TNF-α, presumably via a cyclic GMP-dependent pathway.

【 授权许可】

Unknown