Peroxynitrite stimulates vascular smooth muscle cell cyclic GMP synthesis

FEBS Letters

Ischiropoulos, H.¹  Brock, T.A.²  Gore, J.Z.¹  Beckman, J.S.¹  Tarpey, M.M.¹ 

[1] Department of Anesthesiology, University of Alabama at Birmingham, 952 Tinsley Harrison Tower, Birmingham, AL 35294, USA;Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA

关键词: Peroxynitrite;    Superoxide;    Nitric oxide;    Cyclic GMP;    Endothelial cell;    Free radical;    BAEC;    bovine aortic endothelial cells;    BSO;    buthionine sulfoximine;    cGMP;    cyclic GMP;    ·OH;    hydroxyl radical;    IBMX;    isobutylmethylxanthine;    ·NO;    nitric oxide;    NO3 −;    nitrate;    NO2 −;    nitrite;    ·NO2;    nitrogen dioxide;    HbO2;    oxyhemoglobin;    ONOOH;    peroxynitrous acid;    PBS;    phosphate-buffered saline;    RASM;    rat aortic smooth muscle cells;    SNP;    sodium nitroprusside;    O2 ·−;    superoxide;    SOD;    superoxide dismutase;   

DOI  :  10.1016/0014-5793(95)00413-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Peroxynitrite stimulated the synthesis of cyclic GMP by rat aortic smooth muscle in a time- and dose-dependent manner. Peak formation of cyclic GMP occurred at 1 min with 100 μM peroxynitrite and was inhibited by oxyhemoglobin. Peroxynitrite was less potent than nitric oxide in stimulating cyclic GMP synthesis. Peroxynitrite also enhanced endothelial-dependent cyclic GMP synthesis, via generation of a long-lived substance, which was prevented by inhibition of glutathione synthesis. These data show that peroxynitrite stimulates cyclic GMP synthesis, inferring production of low yields of nitric oxide or associated derivatives. Additionally, vascular exposure to peroxynitrite potentiates endothelial-dependent activation of guanylate cyclase.

【 授权许可】

Unknown