Lipopolysaccharide interaction with hemolin, an insect member of the Ig‐superfamily

FEBS Letters

Faye, Ingrid²  Daffre, Sirlei¹ 

[1] Departamento de Parasitologia, Instituto Ciencias Biomedicas, Universidade Sao Paulo, CEP 05508-900 Cidade Universitaria, Sao Paolo, Brazil;Department of Genetics, Stockholm University, S-10691 Stockholm, Sweden

关键词: Hemolin;    Lipopolysaccharide;    Insect immunity;    Photoaffinity labeling;   

DOI  :  10.1016/S0014-5793(97)00397-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

This study is an attempt to reach some understanding of how insects recognize intruding microorganisms and foreign entities while executing an immune response. We used lipopolysaccharide (LPS) from Escherichia coli, bound to a radiolabeled iodinated crosslinker, to identify hemolymph proteins from the Hyalophora cecropia moth that have the capacity to bind LPS. High amounts of radioactivity were conferred to hemolin, an immunoglobulin and NCAM-related protein, the concentration of which increases in insect hemolymph upon bacterial infection. We could demonstrate a concentration-dependant binding of hemolin to LPS. In addition we could show that Lipid A can compete for this binding, whereas KDO has no effect, indicating that hemolin interacts specifically with the Lipid A moiety of LPS.

【 授权许可】

Unknown